Somatic cell nuclear transfer efficiency: How can it be improved through nuclear remodeling and reprogramming?
نویسندگان
چکیده
منابع مشابه
I-12: Nuclear Reprogramming in Bovin Somatic Cell Nuclear Transfer
Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
متن کاملReprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling.
BACKGROUND Somatic cell nuclear transfer (SCNT) requires cytoplast-mediated reprogramming of the donor nucleus. Cytoplast factors such as maturation promoting factor are implicated based on their involvement in nuclear envelope breakdown (NEBD) and premature chromosome condensation (PCC). Given prior difficulties in SCNT in primates using conventional protocols, we hypothesized that the ability...
متن کاملReprogramming somatic cells: pluripotency through gene induction and nuclear transfer
Background: The understanding of nuclear reprogramming pathways provides important contributions to applied and basic sciences such as the development of autologous cellular therapies for the treatment of numerous diseases, the improved efficiency of animal-based biotechnology or the generation of functional gametes in vitro. Strategies such as nuclear transfer and induced reprogramming have be...
متن کاملEfficiency of Ovine Fibroblast or Cumulus Cells For Somatic Cell Nuclear Transfer in Sheep
Purpose: Despite remarkable progresses have been achieved in the field of somatic cell nuclear transfer (SCNT), there is little information regarding the effect of donor cell type on the efficiency mammalian somatic cell cloning in vitro. This study compared in vitro developmental competency of sheep enucleated oocytes reconstructed with either fibroblast or cumulus cells. Material and methods...
متن کاملEpigenetic reprogramming by somatic cell nuclear transfer in primates.
We recently demonstrated that somatic cells from adult primates could be reprogrammed into a pluripotent state by somatic cell nuclear transfer. However, the low efficiency with donor cells from one monkey necessitated the need for large oocyte numbers. Here, we demonstrate nearly threefold higher blastocyst development and embryonic stem (ES) cell derivation rates with different nuclear donor ...
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ژورنال
عنوان ژورنال: Molecular Reproduction and Development
سال: 2010
ISSN: 1040-452X
DOI: 10.1002/mrd.21242